Carrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown). Creの発現がほぼ全身の臓器・組織に於いて検出される。 B (1-3 months) Developed by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background. <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jul_2013_mm" target="_blank">Mouse of the Month Jul 2013</A> RBRC03801 Villin-NLSCre Villin-NLSCre <a href='https://brc.riken.jp/mus/pcr03801'>Genotyping protocol -PCR-</a> 条件を付加する。<br>研究成果の公表にあたっては、八神健一（筑波大学生命科学動物資源センター）と理研バイオリソースセンターに対して謝辞の表明を必要とする。研究成果の公表にあたって寄託者の指定する文献を引用する(後日指定)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 true Cre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse villin 1 promoter fragment (intestinal stem cells specific promoter region) derived from the BAC library was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community. C57BL/6N-Tg(Vil1-cre)2Utr/Rbrc C57BL/6N-Tg(Vil1-cre)2Utr/Rbrc P1 phage Cre gene, yeast FRT (flipase recombination target) site, Mouse Villin gene promoter (intestinal stem cells specific promoter region), Phage P1 loxP derived from pBACe3.6 vector, Simian virus 40 Large T antigen nuclear localization signal (NLS), poly A Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> RIKEN BRC In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RIKEN BRC B（1〜3か月）